Expert qPCR & dPCR Troubleshooting: Assay Fix Reviews
Strategies to eliminate technical variability and secure reproducible results.
Root Causes of Assay Failure
Primer Dimer Formation
Non-specific amplification can compete with your target sequence, leading to overestimation of low-abundance targets and reduced assay efficiency.
Sample Degradation
Degraded RNA or DNA templates often result in inconsistent Cq values and poor reproducibility, compromising the integrity of longitudinal studies.
Chemical Inhibition
Extraction residuals like phenol or ethanol can inhibit polymerase activity, resulting in suboptimal master mix performance and sensitive assay drift.
Standardizing Success
Our methodology adheres to MIQE standards, ensuring that every qPCR and dPCR assay is optimized for peak performance. We eliminate primer-dimer interference and perform rigorous amplification curve analysis to deliver data that is transparent, reproducible, and ready for publication.
From Failure to Publication
Get expert feedback on your assay data within 24 hours. Our team provides forensic data analysis with actionable strategies when performance matters most.